Patent 12071665 was granted and assigned to Singular genomics Systems, Inc. on August, 2024 by the United States Patent and Trademark Office.
Disclosed herein, inter alia, are compositions and methods for amplification of nucleic acid templates, including hybridizing a linear polynucleotide to an immobilized primer on a surface, circularizing the linear polynucleotide to form a circular polynucleotide, and extending the primer with a polymerase.