Apparatus and methods are provided relating to bottom hole assemblies (BHA) for the isolation and treatment of selected zones using an uphole and a downhole sealing element. The BHA also includes a location and shifting mechanism for locating, engaging and actuating features in a wellbore. The BHA provides for the selective location and actuation of downhole features such as flow valves, precise introduction of fluid, and real-time monitoring of downhole conditions. Methods of deploying a BHA and treating an area of interest using the BHA includes engaging sealing elements using hydraulic pressure.