US Patent 11940381 Calibration of multispectral analysis systems

Methods for calibrating a multispectral analysis system include calibrating the system to detect fluorescence emission from a first fluorescent entity in a biological sample that includes the first fluorescent entity and a second fluorescent entity using a calibration sample, where the calibration sample features a first concentration of the first fluorescent entity and a second concentration of the second fluorescent entity, and where the first concentration is larger than the second concentration.