US Patent 11913052 Cell-free protein synthesis driven metabolic engineering

Disclosed are cell-free systems for metabolic engineering, methods for cell-free metabolic engineering, kits for preparing the disclosed systems, and kits for performing the disclosed methods. The disclosed systems, methods, and kits may be utilized to prepare a chemical product or natural product and to optimize conditions for preparing a chemical product or natural product. The disclosed systems, methods, and kits also may be utilized for combinatorial cell-free metabolism engineering.