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US Patent 11866773 Isolated oligonucleotides containing modified nucleotides

Patent 11866773 was granted and assigned to Envirologix on January, 2024 by the United States Patent and Trademark Office.

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 US Patent 11866773 Isolated oligonucleotides containing modified nucleotides

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Patent abstract

Described and featured herein are isolated oligonucleotides containing, from 5′ to 3′, a nicking enzyme recognition sequence, at least 9 nucleotides that specifically bind a target nucleic acid molecule, and 2′ modified nucleotides. The isolated oligonucleotides may be used in compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a Nicking and Extension Amplification Reaction (NEAR) reaction.

Infobox
Is a
Patent
Patent
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Patent Applicant
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Envirologix
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Current Assignee
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Envirologix
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
118667730
Patent Inventor Names
Daniel Shaffer0
Stephen A. Judice0
Date of Patent
January 9, 2024
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Patent Application Number
175266380
Date Filed
November 15, 2021
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Patent Citations
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US Patent 7094539 None
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US Patent 7112423 Nucleic acid amplification using nicking agents
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US Patent 7282328 Helicase dependent amplification of nucleic acids
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US Patent 7662594 Helicase-dependent amplification of RNA
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US Patent 8574847 Use of blocker oligonucleotides in selective amplification of target sequences
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US Patent 11208687 Compositions and methods for quantifying a nucleic acid sequence in a sample
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US Patent 9096897 Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
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US Patent 9322053 Compositions and methods for monitoring in real time a nicking amplification reaction comprising a primer oligonucleotide with a 3′—terminal region comprising A2′—modified nucleotide
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US Patent 9631231 Compositions and methods for amplifying a nucleic acid sequence comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
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US Patent 9845510 Compositions and methods for rapid detection of
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US Patent 10077467 Compositions and methods for detecting a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
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US Patent 10100370 Compositions and methods for detecting huanglongbing
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US Patent 10584376 Compositions and methods for detecting a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
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US Patent 10793922 Compositions and methods for detecting an RNA virus
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US Patent 6977148 Multiple displacement amplification
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US Patent 7056671 Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
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US Patent 7074600 Amplification of denatured and stabilized nucleic acids
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Patent Primary Examiner
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Kenneth R Horlick
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CPC Code
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C12Q 1/6853
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