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US Patent 11591591 Isolation of high molecular weight DNA using beads

Patent 11591591 was granted and assigned to New England Biolabs on February, 2023 by the United States Patent and Trademark Office.

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Is a
Patent
Patent

Patent attributes

Patent Applicant
New England Biolabs
New England Biolabs
Current Assignee
New England Biolabs
New England Biolabs
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
11591591
Date of Patent
February 28, 2023
Patent Application Number
16547844
Date Filed
August 22, 2019
Patent Primary Examiner
‌
Jill A Warden
CPC Code
‌
C12N 15/101
‌
C12N 15/1013
‌
C12Q 1/6804
‌
C12Q 1/6806
‌
C12N 15/1003
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B01L 3/5021

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 μm in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

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