A thermal image positioning method for determining a heat source location of the thermal image is disclosed. The thermal image positioning method includes obtaining a temperature array corresponding to the thermal image and determining a region of interest (ROI) of the temperature array. The thermal image positioning method further includes determining an ROI center reference point of the ROI, determining a plurality of corner points corresponding to the ROI, and determining the heat source location according to at least one of the plurality of corner points. A thermal image positioning system is also disclosed herein.