A gutter uplift channel assembly is configured to mount skylights, smoke vents, or other roof accessories to a roof having a pair of longitudinally extending roof curbs. A gutter extends between the pair of roof curbs and is secured to the roof curbs to channel water laterally over the roof curbs. The gutter receives longitudinal ends of a pair of roof accessories to be mounted next to each other. An uplift channel or cap of the gutter channel assembly extends over the longitudinal ends or rims of the roof accessories and is secured to the gutter via threaded rods extending up from the gutter through corresponding holes in the uplift channel and nuts placed on the threaded rods.