Methods, systems, and computer readable media for optimizing storage of application data in memory are disclosed. According to one method for optimizing storage of application data in memory, the method includes receiving application data associated with an application. The method also includes generating, using information about the application, information about a processor, and information about a memory, a memory map indicating one or more memory locations in the memory for storing the application data. The method further includes storing, using the memory map, the application data in the one or more memory locations. The method also includes executing, using the processor, the application that uses the application data.