Patent attributes
A computer-implemented system and method for analyzing a biological sample are disclosed. A sequence of images of the biological sample from an image capture device are received from an image capture device, wherein each image of the sequence of images is acquired at a particular focal plane of the biological sample. In addition, an object map is developed from the sequence of images and the object map is analyzed to measure a three-dimensional characteristic of a particular object. The object map comprises a plurality of object map planes, each object map plane is associated with one image of the sequence of images, and pixels of the object map planes associated with the particular object in the sequence of images are assigned a unique identifier associated with the particular object.