Is a
Patent attributes
Patent Applicant
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Richard J. Lawrence0
Linda Rymarquis0
Veena Veena0
Rosemarie Kuehn0
Andrei Y. Kouranov0
Brent Brower-Toland0
Ervin D. Nagy0
Date of Patent
November 30, 2021
0Patent Application Number
151201100
Date Filed
February 27, 2015
0Patent Primary Examiner
Patent abstract
The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.
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