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US Patent 11174494 Expression vector system comprising two selection markers

Patent 11174494 was granted and assigned to Novartis on November, 2021 by the United States Patent and Trademark Office.

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Patent abstractTimelineTable: Further ResourcesReferences
Is a
Patent
Patent
1

Patent attributes

Patent Applicant
Novartis
Novartis
1
Current Assignee
Novartis
Novartis
1
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
1
Patent Number
111744941
Patent Inventor Names
Thomas Jostock1
Hans-Peter Knopf1
Date of Patent
November 16, 2021
1
Patent Application Number
159881411
Date Filed
May 24, 2018
1
Patent Primary Examiner
‌
Antonio Galisteo Gonzalez
1
Patent abstract

The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least(a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest;(b) a polynucleotide encoding a first selectable marker (sm I);(c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I),wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

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