US Patent 11118213 Optimization of multigene analysis of tumor samples

A method of analyzing a tumor sample comprising:(a) acquiring a library comprising a plurality of tumor members from a tumor sample;(b) contacting the library with a bait set to provide selected members;(c) acquiring a read for a subgenomic interval from a tumor member from said library;(d) aligning said read; and(e) assigning a nucleotide value (e.g., calling a mutation) from said read for the preselected nucleotide position, thereby analyzing said tumor sample.