Patent attributes
The present invention provides a DNA barcode and method for identifying cuttlefishes, mainly depending on the diversity of mitochondrial DNA, including designing universal primers on conservative regions on both sides of the species-specific variable region, amplifying DNA from cuttlefishes, and sequencing PCR products to obtain DNA sequences, and identifying cuttlefish species based on the similarity by comparing with the species information in the database. The method is advantageous in that it can achieve efficient and specific amplification of DNA from such cephalopods as cuttlefishes, with low requirements for necessary instruments and simple operation processes. In addition, the method can be carried out in most molecular biology laboratories, which greatly improves the probability of success of molecular identification for cuttlefishes and sibling species thereof.
