Is a
Patent attributes
Patent Applicant
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Andrew Lees0
Min-Ju Chang0
Natalia Oganesyan0
Date of Patent
July 13, 2021
0Patent Application Number
168197750
Date Filed
March 16, 2020
0Patent Citations Received
Patent Primary Examiner
Patent abstract
The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5′-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.
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