US Patent 10964001 Multispectral imaging systems and methods

The methods and systems disclosed herein include obtaining a first image of a sample, where the first image corresponds to light transmitted through the sample in a first plurality of distinct spectral bands, obtaining a second image of the sample, where the second image corresponds to light transmitted through the sample in a second plurality of distinct spectral bands, and where at least some members of the second plurality of spectral bands are different from the members of the first plurality of spectral bands, and combining the first and second images to form a multispectral image stack, where each pixel in the image stack includes information corresponding to at least four distinct spectral bands, and where the at least four distinct spectral bands include at least one member from the first plurality of spectral bands and at least one member from the second plurality of spectral bands.