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US Patent 10563226 Enhancing endonuclease based gene editing in primary cells

Patent 10563226 was granted and assigned to Seattle Children's on February, 2020 by the United States Patent and Trademark Office.

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Patent
Patent
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Patent attributes

Patent Applicant
Seattle Children's
Seattle Children's
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Current Assignee
Seattle Children's
Seattle Children's
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
105632260
Patent Inventor Names
Michael C. Jensen0
Andrew Scharenberg0
David Rawlings0
Kamila Gwiazda0
Alexandra Grier0
Date of Patent
February 18, 2020
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Patent Application Number
151529600
Date Filed
May 12, 2016
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Patent Primary Examiner
‌
Celine X Qian
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Patent abstract

Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.

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