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US Patent 10240146 Probe library construction

Patent 10240146 was granted and assigned to President and Fellows of Harvard College on March, 2019 by the United States Patent and Trademark Office.

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Patent
Patent
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Patent attributes

Current Assignee
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President and Fellows of Harvard College
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
102401460
Patent Inventor Names
Jeffrey R. Moffitt0
Xiaowei Zhuang0
Alistair Boettiger0
Date of Patent
March 26, 2019
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Patent Application Number
153296510
Date Filed
July 29, 2015
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Patent Citations Received
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US Patent 11788123 Systems and methods for high-throughput image-based screening
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US Patent 12116625 Barcode diffusion-based spatial omics
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US Patent 12104151 Systems and methods for determining nucleic acids
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US Patent 11959075 Systems and methods for determining nucleic acids
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Patent Primary Examiner
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Jeremy C Flinders
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Patent abstract

The present invention generally relates to systems and methods for producing nucleic acids. In some aspects, relatively large quantities of oligonucleotides can be produced, and in some cases, the oligonucleotides may have a variety of different sequences and/or lengths. For instance, a relatively small quantity of oligonucleotides may be amplified to produce a large amount of nucleotides. In one set of embodiments, oligonucleotides may be amplified using PCR, then transcribed to produce RNA. The RNA may then be reverse transcribed to produce DNA, and optionally, the RNA may be selectively degraded or removed, relative to the DNA. In one set of embodiments, the oligonucleotides may be chemically modified. These modifications may include, but are not limited, to the adding of fluorescent dyes or other signaling entities.

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