Is a
Patent attributes
Patent Applicant
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Scott D. Rose0
Susan M. Rupp0
Joseph Alan Walder0
Joseph R. Dobosy0
Mark Aaron Behlke0
Date of Patent
March 12, 2019
0Patent Application Number
141239440
Date Filed
December 2, 2013
0Patent Citations Received
Patent Primary Examiner
Patent abstract
Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.
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