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US Patent 10095832 Strategies for high throughput identification and detection of polymorphisms

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Patent abstractTimelineTable: Further ResourcesReferences
Is a
Patent
Patent
1

Patent attributes

Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
1
Patent Number
100958321
Patent Inventor Names
Michael Josephus Theresia Van Eijk1
Henricus Johannes Adam Van Der Poel1
Date of Patent
October 9, 2018
1
Patent Application Number
158629561
Date Filed
January 5, 2018
1
Patent Citations Received
‌
US Patent 11499188 Nucleic acid constructs and methods of use
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US Patent 11499187 Nucleic acid constructs and methods of use
‌
US Patent 11505828 Methods for spatial analysis using RNA-templated ligation
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US Patent 12129516 Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
8
‌
US Patent 11479809 Methods of detecting analytes
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US Patent 11479810 Spatially encoded biological assays
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US Patent 11492612 Methods of determining a surgical margin and methods of use thereof
‌
US Patent 12071655 Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
13
...
Patent Primary Examiner
‌
Cynthia B Wilder
1
Patent abstract

The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

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