An array substrate and a fabrication method thereof, and a display device are provided. The array substrate includes a base substrate and a peripheral lead, a ground signal terminal, a conductive adhesive, and a barrier wall structure located on the base substrate, wherein, the conductive adhesive is connected with the ground signal terminal, and the barrier wall structure is located between the peripheral lead and the ground signal terminal, and configured for blocking the conductive adhesive from diffusing toward the peripheral lead.