SBIR/STTR Award attributes
The threat of organophosphate (OP) chemical warfare agents (CWA) to United States military personnel is a persistent problem due to the relative ease of manufacturing chemical threats compared to nuclear and biological agents. Human butyrylcholinesterase (HuBChE) has been identified as a probable bio-scavenger of OP CWAs that could be used to protect individuals that could be exposed to OP CWAs. The goal of this project is to generate a mammalian cell line capable of expressing human HuBChE in tetrameric form with appropriate human glycosylation patterns . To achieve this goal we will down select among our multiple HuBChE expressing cell lines and humanize the observed glycosylation patterns to match the plasma derived HuBChE standard. This approach will be evaluated to determine technical and economic applicability to full scale manufacturing.
