Recombineering is a method of genetic engineering in bacterial genomes that involves homologous recombination. In CRISPR-assisted recombineering, CRISPR assists the selection of successful recombineering events which change the target sequence and negatively selects cells in which the desired homologous recombination has not occurred. Negative selection occcurs by CRISPR-Cas9 induced double stranded breaks which are lethal in bacteria where non homologous end joining (NHEJ) is not very effective.
Documentaries, videos and podcasts
- CRISPR/Cas ToolsClustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated (Cas) proteins perform adaptive immune functions in prokaryotic organisms defending against foreign nucleic acids such as viruses. CRISPR/Cas tools have been adapted for use in genome editing and other DNA and RNA targeting applications.
- Cluster: BiotechnologyA cluster of topics related to biotechnology.
- Cluster: Synthetic biologyA cluster of topics related to synthetic biology.