Recombineering

A method of genetic engineering in bacterial genomes that involves homologous recombination.

Recombineering is a method of genetic engineering in bacterial genomes that involves homologous recombination. In CRISPR-assisted recombineering, CRISPR assists the selection of successful recombineering events which change the target sequence and negatively selects cells in which the desired homologous recombination has not occurred. Negative selection occcurs by CRISPR-Cas9 induced double stranded breaks which are lethal in bacteria where non homologous end joining (NHEJ) is not very effective.

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