Log in
Enquire now
PCR (polymerase chain reaction)

PCR (polymerase chain reaction)

PCR is used to amplify or generate many copies of a target sequence of DNA.

OverviewStructured DataIssuesContributors

Contents

Polymerase chain reaction is a method of amplifying or generating many copies of a target region of DNA. PCR reactions are normally carried out in a thermocycler which can rapidly change the temperature of the reaction which take place in 3 steps repeated many times. High temperature is applied to double-stranded DNA to separate the two strands, a process called denaturation. Lowering the temperature allows primers to bind to the template which is called the annealing step. Primers are short nucleotide sequences, single-stranded DNA, that are complementary to a region on the template DNA. In DNA adenine pairs with thymine (A-T) and cytosine pairs with guanine (C-G). Next is the extension phase where the DNA polymerase adds nucleotides onto the 3’-OH at the end of the primer. From the primer the nucleotide sequence extends as DNA polymerase adds nucleotides are complementary to the template. PCR reactions are exponential and after 35 cycles 236 copies are produced. Reverse transcription PCR (RT-PCR) is a version of PCR that detects RNA by starting with a reverse transcription step to convert RNA into a DNA copy (cDNA), followed by PCR amplification of the cDNA.

Timeline

No Timeline data yet.

Further Resources

Title
Author
Link
Type
Date
No Further Resources data yet.

References

Find more entities like PCR (polymerase chain reaction)

Use the Golden Query Tool to find similar entities by any field in the Knowledge Graph, including industry, location, and more.
Open Query Tool
Access by API
Golden Query Tool
Golden logo

Company

  • Home
  • Press & Media
  • Blog
  • Careers
  • WE'RE HIRING

Products

  • Knowledge Graph
  • Query Tool
  • Data Requests
  • Knowledge Storage
  • API
  • Pricing
  • Enterprise
  • ChatGPT Plugin

Legal

  • Terms of Service
  • Enterprise Terms of Service
  • Privacy Policy

Help

  • Help center
  • API Documentation
  • Contact Us
By using this site, you agree to our Terms of Service.