Leiden Measurement Technology LLC SBIR Phase II Award, August 2021

Leiden Measurement Technolgoy, LLC (LMT) proposes to design and construct the Flat-field Automated UltraViolet Exploration (FAUVE) microscope, a high-resolution, compact, fully-automated epifluorescence microscope operating through the DUV-VIS with sub-micron, high-NA, and a flat field throughout the DUV-VIS. nbsp;The core of FAUVE is a novel DUV-VIS objective, capable of producing sharp images at high magnification with very little chromatic aberration using only materials that are radiation-hard (exceeding 300 krad). Additionally, FAUVE will feature a new microfluidic cartridge platform which will enable the rapid mounting of samples for microscopic viewing. An automated microfluidic subsystem will autonomously filter samples into the disposable cartridges and treat them with user-defined reagents which could include structural stains/dyes or even functionalized suspension array particles. An entirely custom, miniaturized microscope system will be developed and occupy volume of less than 2,000 cc, while still enabling sub-micron imaging throughout the DUV-VIS in up to five different excitation wavelengths.