CRISPR sequence
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats and a CRISPR sequence refers to a DNA locus with hallmark features including, repeated palindromic sequences, which is part of an adaptive immune system in bacteria and a system that has been adapted for use in genome editing applications.
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Validating edits is especially important in the CRISPR experimental process. Next-generation sequencing (NGS) is an easy and high-throughput method to check for desired mutations since off-target mutation and not an edit in the target gene could happen. CRISPR amplicon sequencingamplicon sequencing has become a standardized validation technique for academia, clinic, and industry. High-throughput CRISPR screening is based on the principle of targeted amplicon sequencing by conducting PCR experiments using primers flanking the target region. Targeted amplicon sequencing is the most sensitive method for checking mutations and the detection frequencies are as low as 0.01%.
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