CRISPR-Cas12d

CRISPR-Cas12d belongs to the Type V CRISPR systems, where Cas12d (formerly known as CasY) is a Class II effector protein. The most widely known Class II effector protein, Cas9 in the Type II system binds and cleaves double-stranded DNA targets that match its guide crRNA and has been adapted as a genome editing tool. Cas12d is less well studied but has been shown to be capable of RNA-guided DNA targeting by demonstrating guide RNA-guided DNA interference activity. Cas12d, originally identified as CasY, was pulled out of a search through DNA sequencing data from uncultivated natural microbial communities . Cas12e (formerly CasX) was identified in this same study.

CRISPR-Cas12d belongs to the Type V CRISPR systems, where Cas12d (formerly known as CasY) is thea Class II effector protein. The most widely known Class II effector protein, Cas9 in the Type II system binds and cleaves double-stranded DNA targets that match its guide crRNA and has been adapted as a genome editing tool. Cas12d is less well studied but has been shown to be capable of RNA-guided DNA targeting by demonstrating guide RNA-guided DNA interference activity. Cas12d, originally identified as CasY, was pulled out of a search through DNA sequencing data from uncultivated natural microbial communities .

CRISPR-Cas12d belongs to the Type V CRISPR systems, where Cas12d (formerly known as CasY) is the effector protein. The most widely known effector protein, Cas9 in the Type II system binds and cleaves double-stranded DNA targets that match its guide crRNA. Cas12d is less well studied but has been shown to be capable of RNA-guided DNA targeting by demonstrating guide RNA-guided DNA interference activity. Cas12d, originally identified as CasY, was pulled out of a search through DNA sequencing data from uncultivated natural microbial communities .