Patent 7732565 was granted and assigned to NEW ENGLAND BIOLABS, INC. on June, 2010 by the United States Patent and Trademark Office.
Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.