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US Patent 7416847 In vitro peptide or protein expression library

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Patent attributes

Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
7416847
Patent Inventor Names
Morten Isaksen1
Bjorn H. Lindqvist1
David Andrews1
Elizabeth Haggard-Ljungquist1
Date of Patent
August 26, 2008
Patent Application Number
09331808
Date Filed
February 18, 1998
Patent Primary Examiner
‌
T. D. Wessendorf
Patent abstract

The present invention relates to methods of producing peptide or protein expression libraries. In such a library, a population of nucleotide sequences is expressed. The resulting peptides or proteins encoded by those nucleotide sequences are then screened to identify those peptides or proteins having a desired property such as the ability to bind a selected ligand. In the construction and screening of such libraries, it is desired to ensure that the nucleotide encoding a particular protein remains connected in some way with that protein so that once a desired protein having a selected property has been isolated, its encoding nucleotide sequence may be specifically recovered for subsequent manipulation for example using PCR or sequencing. The present invention makes use of a property not previously described in constructing an expression library namely the use of proteins which covalently bind DNA. DNA is expressed in such a way that the protein so expressed binds to its own encoding DNA therefore allowing the DNA to be associated only with its encoding protein.

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