RNA sequencing techniques are used to determine the sequence of nucleotide bases, adenine (A), cytosine (C), guanine (G) and uracil (U) in RNA molecules. Uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell.
RNA sequencing is replacing gene expression arrays to analyse the spectrum and abundance of transcripts in a given cell or tissue type at a given time. The technique called RNA-Seq, also known as whole transcriptome shotgun sequencingshotgun sequencing generates cDNA and uses it in next-generation sequencing.
