The determination of the sequence of nucleic acids in genomic DNA of bacteria using DNA sequencing techniques
Currently, the complete sequence map of more than 90% bacterial strains can be constructed by making use of a combination of Illumina HiSeq and PacBio SMRT systems. Pacbio RS II system can achieve complete genome assembly even in the regions of high or low GC content, as well as repetitive sequences. The complete sequence map of the rest 10% bacterial strains can be achieved with Sanger sequencing data. CD GenomicsCD Genomics has completed hundreds of bacterial genome assembly cases without gap.