He first became famous as an impersonator and is known for his talent at parody and his duets with the famous Russian pop singer Alla Pugacheva and Ani Lorak.
Galkin was also the host of the Russian version of Who Wants to Be a Millionaire?, after it was renamed from O, Schastlivchik (Oh, Lucky Man) and to 2008 and host celebrity episode of 25 December 2002 Russian Roulette He currently hosts the Russian adaptation of The Million Pound Drop.
During his University years Galkin appeared in KVN, the Russian youth stand-up comedy competition, which kickstarted his career. Recently, he acted as a judge on the show.
Galkin and Pugacheva are hosts of a Russian TV show similar to But Can They Sing?, Dve Zvezdy (Two Stars). Pairs made up of a professional singer and a celebrity compete for the honour of being named Two Stars. Famous alumnae include the singers Zhasmin, Alsou and Nastya Kamenskikh.
Galkin had a very classical and strict upbringing. His mother was a teacher, and he finished music and math school, and knows four languages, Russian, English, German and French. He is a graduate of the Russian State University of the Humanities. Although he pursued a PhD at the university, he abandoned his studies with a master's degree in linguistics to pursue comedy.
Galkin is one of the few comedians currently performing an impression, albeit 'light-hearted', of President Vladimir Putin, on Russia's Channel One TV channel.
Since November 2016 he has been hosting a children's talent show on TV "Best of all".