Cell lines are used in recombinant protein and monoclonal antibody production, drug screening, and gene functional studies. The process of developing stable cell lines starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated.
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