Single-Cell RNA Sequencing
Single Cell RNA Seq
CD Genomics provides robust transcriptome research service down to single-cell input levels in high-quality samples These global gene expression patterns in single cells already have dramatically advanced cell biology.
Single-cell RNA-Seq can be used to examine the expression of individual cells and provides a higher resolution of cellular differences as compared to traditional RNA-Seq. Single-cell RNA-Seq enables us to understand the function of an individual cell in the context of its microenvironment.
Cells are the basic unit of life and each cell is unique. The ability to reveal complex cellular events in biological systems is critical to a better understanding of cellular contributions during development or in disease progression. Gene expression research at the single-cell resolution on samples composed of mixed cell populations allows for deep insight into c into the transcriptome complexity of diverse cell types.
The advent of cell sorting/partitioning technologies, such as flow cytometry and microfluidics, has made it possible to capture single cells, and the DNA or RNA of single cells is amplified for single-cell sequencing.
Cells are the basic unit of life. The ability to reveal complex cellular events in biological systems is critical to a better understanding of cellular contributions during development or in disease progression. Single-cell RNA-Seq can be used to examine the gene expression of individual cells and provides a higher resolution of cellular differences as compared to traditional RNA-Seq. Single-cell RNA-Seq enables us to understand the function of an individual cell in the context of its microenvironment.
Fluidigm C1 Single-Cell mRNA Workflow. With Fluidigm C1 system, We provide single-cell transcriptome profiling service at an optional scale. C1 can rapidly and reliably capture and process individual cells. The steps in Integrated C1 Single-Cell mRNA Seq workflow include fluidics circuits (IFCs) to capture cells, convert polyA+ RNA into full-length cDNA, and perform universal amplification of the cDNA. With the customizable microfluidic circuits, C1 enables seamlessly transition from identifying critical cell populations to generate sequencing libraries for transcript 3′ End Counting, full-length mRNA sequencing, DNA sequencing, epigenetic analysis, micro-RNA expression profiling and more.