Philip Sebastian

Better Belly is a mobile app service to help you live a healthier, productive, positive life one food entry at a time.

Better Belly is a mobile app service to help you live a healthier, productive, positive life one food entry at a time..

Founded in 2017, Beacon VC is a wholly-owned corporate venture capital fund of Kasikornbank PLC, a commercial bank in ThailandThailand. With an initial fund of $30 million, Beacon VC focuses on strategic investments in early to growth-stage technology startups covering not only financial technology but also consumer internet and enterprise technology such as artificial intelligence (AI) and enterprise IT. In 2018, Beacon VC expanded its fund size to $135 million.

Venturepreneurs Organisations is an investment fund that specializes in the seed-stage venture capital investments. The firm prefers to invest in the business products, consumer products and information technology sectors. It is based in Barcelona, SpainSpain. Venturepreneurs was founded by Christopher Pommerening in 2007. They offered a peer-to-peer network to provide European entrepreneurs a place to exchange knowledge and experience.

Roman Vasilenko was born on 5 April 1969 in Leningrad (USSR). Roman's parents were officers of naval aviation, that is why in 1990 he graduated Yaroslavl Higher Military Financial School where he received his military education. After the studying and until 1998, he served in the Leningrad Naval Base. He also received an MBA in FranceFrance, Austria, Hungary, Switzerland and the United States.

The identification and quantification of proteins lags behind DNA-sequencing methods in scale, sensitivity, and dynamic range. Here, we show that sparse amino acid-sequence information can be obtained for individual protein molecules for thousands to millions of molecules in parallel. We demonstrate selective fluorescence labeling of cysteine and lysine residues in peptide samples, immobilization of labeled peptides on a glass surface, and imaging by total internal reflection microscopy to monitor decreases in each molecule's fluorescence after consecutive rounds of Edman degradation. The obtained sparse fluorescent sequence of each molecule was then assigned to its parent protein in a reference database. We tested the method on synthetic and naturally derived peptide molecules in zeptomole-scale quantities. We also fluorescently labeled phosphoserines and achieved single-molecule positional readout of the phosphorylated sites. We measured >93% efficiencies for dye labeling, survival, and cleavage; further improvements should enable studies of increasingly complex proteomic mixtures, with the high sensitivity and digital quantification offered by single-molecule sequencing.

We thank B. Cannon and R. Russell for early assistance with single-molecule imaging, M. Gadush for assistance with peptide synthesis, I. Riddington, J. Dinser, and K. Suhr for assistance in mass spectrometry analysis of fluorescently labeled peptides, Z. Simpson and J. Rybarski for assistance with image analysis, A. Ellington for many fruitful discussions, and the Texas Advanced Computing Center for high-performance computing. This work was supported by fellowships from the HHMI (to J.S.) and NSF (DGE-1610403 to A.A.B.), and by grants from DARPA (N66001-14-2-4051 to E.V.A. and E.M.M.), NIH (DP1 GM106408, R01 GM076536, and R35 GM122480 to E.M.M.), CPRIT (to E.M.M.), and the Welch foundation (F-1515 to E.M.M. and F-0046 to E.V.A.).

J.S., A.A.B., E.T.H., A.M.B., J.L.B., A.M.J., E.V.A., and E.M.M. designed and analyzed the experiments or interpreted the data. J.S., E.T.H., A.M.B., J.L.B., and J.M. performed the experiments. J.S., A.A.B., E.T.H., A.M.B., E.V.A., and E.M.M. wrote and edited the manuscript.

J.S., A.M.B., E.M.M., and E.V.A. are cofounders and shareholders of Erisyon Inc. J.S., E.M.M., and E.V.A. are co-inventors on granted US patent PCT/US2012/043769. J.S., A.A.B., E.T.H., J.L.B., A.M.J., E.V.A., and E.M.M. are co-inventors on pending US patent PCT/US2015/050099.

Full one- and two-dye fluorosequencing histograms for peptides in Fig. 5a and Fig. 5d confirm signal for the expected sequence patterns.

The identification and quantification of proteins lags behind DNA-sequencing methods in scale, sensitivity, and dynamic range. Here, we show that sparse amino acid-sequence information can be obtained for individual protein molecules for thousands to millions of molecules in parallel. We demonstrate selective fluorescence labeling of cysteine and lysine residues in peptide samples, immobilization of labeled peptides on a glass surface, and imaging by total internal reflection microscopy to monitor decreases in each molecule's fluorescence after consecutive rounds of Edman degradation. The obtained sparse fluorescent sequence of each molecule was then assigned to its parent protein in a reference database. We tested the method on synthetic and naturally derived peptide molecules in zeptomole-scale quantities. We also fluorescently labeled phosphoserines and achieved single-molecule positional readout of the phosphorylated sites. We measured >93% efficiencies for dye labeling, survival, and cleavage; further improvements should enable studies of increasingly complex proteomic mixtures, with the high sensitivity and digital quantification offered by single-molecule sequencing.

We thank B. Cannon and R. Russell for early assistance with single-molecule imaging, M. Gadush for assistance with peptide synthesis, I. Riddington, J. Dinser, and K. Suhr for assistance in mass spectrometry analysis of fluorescently labeled peptides, Z. Simpson and J. Rybarski for assistance with image analysis, A. Ellington for many fruitful discussions, and the Texas Advanced Computing Center for high-performance computing. This work was supported by fellowships from the HHMI (to J.S.) and NSF (DGE-1610403 to A.A.B.), and by grants from DARPA (N66001-14-2-4051 to E.V.A. and E.M.M.), NIH (DP1 GM106408, R01 GM076536, and R35 GM122480 to E.M.M.), CPRIT (to E.M.M.), and the Welch foundation (F-1515 to E.M.M. and F-0046 to E.V.A.).

J.S., A.A.B., E.T.H., A.M.B., J.L.B., A.M.J., E.V.A., and E.M.M. designed and analyzed the experiments or interpreted the data. J.S., E.T.H., A.M.B., J.L.B., and J.M. performed the experiments. J.S., A.A.B., E.T.H., A.M.B., E.V.A., and E.M.M. wrote and edited the manuscript.

J.S., A.M.B., E.M.M., and E.V.A. are cofounders and shareholders of Erisyon Inc. J.S., E.M.M., and E.V.A. are co-inventors on granted US patent PCT/US2012/043769. J.S., A.A.B., E.T.H., J.L.B., A.M.J., E.V.A., and E.M.M. are co-inventors on pending US patent PCT/US2015/050099.

Full one- and two-dye fluorosequencing histograms for peptides in Fig. 5a and Fig. 5d confirm signal for the expected sequence patterns.

The company's full legal name is Gattaka Inc. Inspired by the science fiction movie Gattaca with Ethan Hawke and Uma Thurman.

Favorite quotes:

• Blood has no nationality. ~Vincent
• We shed 500 million cells a day. ~Vincent
• You want to know how I did it? This is how I did it, Anton: I never saved anything for the swim back. ~Vincent

Tezos is a decentralized blockchain whose protocol allows for stakeholders to approve any amendments to the protocol led by Kathleen and Arthur Breitman. It is a platform for smart contracts which facilitates formal verification of the mathematical properties of the code governing transactions with the intention of being a true digital commonwealth. The currency the native to the Tezos blockchain is called tezzie (XTZ). Governance of Tezos is designed to be a commonwealth such that token holders vote and determine future upgrades to the protocol. This is called on-chain governance, which prioritizes voting for soft-fork upgrades to the network rather than the network splits that occur via hard forks in other blockchain networks like Bitcoin and EthereumEthereum.