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US Patent 8932814 CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes

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Is a
Patent
Patent
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Patent attributes

Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
89328140
Patent Inventor Names
Feng Zhang0
Le Cong0
Date of Patent
January 13, 2015
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Patent Application Number
142584580
Date Filed
April 22, 2014
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Patent Citations Received
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US Patent 12123032 CRISPR enzyme mutations reducing off-target effects
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US Patent 12110500 Universal donor stem cells and related methods
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US Patent 12110545 Methods of assessing nuclease cleavage
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US Patent 12116619 CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
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US Patent 12121524 DNA-PK inhibitors
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US Patent 12123015 Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
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US Patent 11713485 Hybridization chain reaction methods for in situ molecular detection
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US Patent 11773411 Chimeric proteins and methods of regulating gene expression
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Patent Primary Examiner
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Anne Gussow
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Patent abstract

The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

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