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US Patent 11353448 Methods and compositions for quantifying metabolites and proteins from single cells

Patent 11353448 was granted and assigned to California Institute of Technology on June, 2022 by the United States Patent and Trademark Office.

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Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.

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