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US Patent 10059990 In situ nucleic acid sequencing of expanded biological samples

Patent 10059990 was granted and assigned to Massachusetts Institute of Technology on August, 2018 by the United States Patent and Trademark Office.

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Current Assignee
Massachusetts Institute of Technology
Massachusetts Institute of Technology
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
100599900
Patent Inventor Names
George Church0
Edward Stuart Boyden0
Evan Daugharthy0
Fei Chen0
Paul Warren Tillberg0
Shahar Alon0
Adam Marblestone0
Date of Patent
August 28, 2018
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Patent Application Number
150989680
Date Filed
April 14, 2016
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Patent Citations Received
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US Patent 12129516 Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
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US Patent 11492662 Methods for in situ transcriptomics and proteomics
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US Patent 11501440 Systems and methods for spatial analysis of analytes using fiducial alignment
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US Patent 11505828 Methods for spatial analysis using RNA-templated ligation
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US Patent 12071655 Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
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US Patent 12076701 Capturing oligonucleotides in spatial transcriptomics
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US Patent 12112833 Systems and methods for index hopping filtering
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US Patent 12110541 Methods for preparing high-resolution spatial arrays
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Patent Primary Examiner
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Karla A Dines
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Patent abstract

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).

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