RNA sequencing techniques are used to determine the sequence of nucleotide bases, adenine (A), cytosine (C), guanine (G) and uracil (U) in RNA molecules.
RNA sequencing is replacing gene expression arrays to analyse the spectrum and abundance of transcripts in a given cell or tissue type at a given time. The technique called RNA-Seq, also known as whole transcriptome shotgun sequencingshotgun sequencing generates cDNA and uses it in next-generation sequencing.