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Programmed chromosome fission

Programmed chromosome fission

A technique for splitting apart chromosomes and large genome fragments in Escherichia coli that utilizes CRISPR-Cas9.

All edits by  Shahid Fakih 

Edits on 16 Sep, 2019
Shahid Fakih
Shahid Fakih approved a suggestion from Golden's AI on 16 Sep, 2019
Edits made to:
Article (+3/-3 characters)
Article

The programmed chromosome fission technique adapts CRISPR-Cas9 to excise long stretches without leaving unwanted errors of “genetic scars”. After a segment of DNADNA is excised, the programmed chromosome fusion technique uses an altered version of lambda red recombinase to join the ends of both the original chromosome and the excised piece to form two circular DNA molecules that are protected form endonucleases. The smaller DNA segments will be easier to make changes to and they can later be combined back together.

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