Golden
Pacific Biosciences

Pacific Biosciences

A biotechnology company founded in 2004 that develops and manufactures systems for gene sequencing and some novel real time biological observation

Single Molecular Real-Time (SMRT) sequencing employs a specialized flow cell with many thousands of individual picolitre wells with transparent bottoms -- zero-mode waveguides (ZMW). The polymerase is fixed to the bottom of the well and allows the DNA strand to progress through the ZMW. As a result, the system can focus on a single molecular. SMRT sequencing allows for real-time imaging of fluorescently tagged nucleotides that are synthesized along individual DNA template molecules. The sequencing reaction ends when the template and polymerase dissociate. The average read length from the PacBio instrument is approximately 2 kb, and some reads may be over 20 kb. Longer reads are especially useful for de novo assemblies of novel genomes that can span many more repeats and bases.



Highly repetitive elements found in both eukaryotic and prokaryotic genomes pose a challenge for genome assembly and make the detailed study of repetitive sequences difficult. Long-read sequencing delivers reads in excess of several or dozens of kilobases (kbs), which can span complex or repetitive regions with a single continuous read, allowing for the resolution of these large structural features. Besides considerably longer and highly accurate DNA sequences from individual unamplified molecules, it can also exhibit where methylated bases occur, thereby providing functional information about DNA methyltransferases encoded by the genome.

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Name
Role
LinkedIn

Aaron Holiday

Investor



Aaron Talebi

Employee



Aiyesha Ma

Employee



Alex Gottschalk

Employee



Alex Hutcheson

Employee



Alvin Lin

Investor



Aniket Deshpande

Employee



ankit master

Employee



Arianna Gianola

Employee



Asif Khalak

Employee



Ben Lerch

Employee



Ben Voiles

Employee



Bryan Shieh

Employee



Casey Marshall

Employee



Chris Kurdziel

Investor



CJ Halabi

Investor



Craig Hamasaki

Employee



Dana Underwood

Employee



Darisha Jhutty

Employee



Davian Contreras

Employee



David Evans

Employee



David Hsu

Employee



David Singer

Investor



Dimitris Iliopoulos

Employee



Donald Gray

Employee



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