The exposition of the museum is organized according to the historical and chronological principle. It presents exhibits related to medicine and health care in the city of Chelyabinsk, archival materials, starting from the appearance of the first medical workers in the city in the 18th century. A significant amount of the exposition is occupied by the period of the 30s of the twentieth century, when many medical and preventive institutions began to appear in the city at enterprises under construction. A separate block is devoted to the period of the Great Patriotic War and the participation of medical workers in it. The work of evacuation hospitals deployed during the war in Chelyabinsk is reflected.