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Feeder-free culture

Feeder-free culture

Cell culture without the use of feeder cells.

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Edits on 22 Mar 2019
Dawson Sewell
Dawson Sewell approved a suggestion from Golden's AI on 22 Mar 2019 1:14 pm
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Matrigel is an extracellular matrix product sold by Corning Life Sciences and Geltrex is a similar product sold by ThermoFisher Scientific. Feeder-free culture systems are being developed for clinical use to avoid patient exposure to animal pathogens.Clinical use of stem cellsstem cells also aims for xeno-free culturing and since Matrigel and Geltrex are extracted from the sarcomas of Engelbreth-Holm-Swarm mice, they are not xeno-free and contain undefined components.

Edits on 19 Mar 2019
Dawson Sewell
Dawson Sewell approved a suggestion from Golden's AI on 19 Mar 2019 10:48 pm
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Some cells require the presence of feeder cells or contact with feeder cells to grow. Instead of feeder cells, certain cell typescell types can be grown on culture surfaces coated with extracellular matrix proteins such as laminin, collagen, fibronectin or on Matrigel, which is a mixture of extracellular matrix components. Some cells may use cell culture medium conditioned by feeder cells. 

Edits on 6 Feb 2019
Jude Gomila
Jude Gomila approved a suggestion from Golden's AI on 6 Feb 2019 12:41 am
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Some cells require the presence of feeder cells or contact with feeder cells to grow. Instead of feeder cells, certain cell types can be grown on culture surfaces coated with extracellular matrix proteinsextracellular matrix proteins such as laminin, collagen, fibronectin or on Matrigel, which is a mixture of extracellular matrix components. Some cells may use cell culture medium conditioned by feeder cells. 

Edits on 5 Feb 2019
Meredith Hanel
Meredith Hanel edited on 5 Feb 2019 3:11 am
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Meredith Hanel
Meredith Hanel edited on 5 Feb 2019 2:56 am
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Matrigel is an extracellular matrix product sold by Corning Life Sciences and Geltrex is a similar product sold by ThermoFisher Scientific. 

Matrigel is an extracellular matrix product sold by Corning Life Sciences and Geltrex is a similar product sold by ThermoFisher Scientific. Feeder-free culture systems are being developed for clinical use to avoid patient exposure to animal pathogens.Clinical use of stem cells also aims for xeno-free culturing and since Matrigel and Geltrex are extracted from the sarcomas of Engelbreth-Holm-Swarm mice, they are not xeno-free and contain undefined components.



Companies selling feeder-free culture products

Stem Cell Technologies (RSeT™ Feeder-Free Medium)

Corning Life Sciences (Matrigel)

Meredith Hanel
Meredith Hanel edited on 5 Feb 2019 2:29 am
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Some cells require the presence of feeder cells or contact with feeder cells to grow. CertainInstead of feeder cells, certain cell types can be grown on culture surfaces coated with extracellular matrix proteins such as laminin, collagen, fibronectin or on Matrigel, which is a mixture of extracellular matrix components. TheseSome cells may also use cell culture medium conditioned by feeder cells. 



Matrigel is an extracellular matrix product sold by Corning Life Sciences and Geltrex is a similar product sold by ThermoFisher Scientific. 

Meredith Hanel
Meredith Hanel edited on 5 Feb 2019 2:14 am
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Feeder-free culture

Cell culture without the use of feeder cells.

Article

Some cells require the presence of feeder cells or contact with feeder cells to grow. Certain cell types can be grown on culture surfaces coated with extracellular matrix proteins such as laminin, collagen, fibronectin or on Matrigel, which is a mixture of extracellular matrix components. These cells may also use cell culture medium conditioned by feeder cells. 

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Meredith Hanel"Initial topic creation"
Meredith Hanel created this topic on 5 Feb 2019 1:43 am
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 Feeder-free culture

Cell culture without the use of feeder cells.

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