Epigenetic CRISPR/Cas9

Epigenetic CRISPR/Cas9

Epigenetic CRISPR/Cas9 is a modified form of the gene editing technology CRISPR, designed to modify the epigenome, rather than the genome. Without cutting DNA the system activates or represses genes.

All edits by  Carla Faraguna 

Edits on 25 Oct, 2018
Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 25 Oct, 2018
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Epigenetic CRISPR-Cas9 is a modified form of the genome editing platform CRISPR-Cas9 that does not cut DNA but affects gene expression levels. Gene expression levels depend on DNA methylation levels and whether chromatin conformation is in an active or repressed state. Epigenetic CRISPR-Cas9 uses the nuclease dead Cas9 (dCas9) to target the genome and instead of altering the genetic sequence, the system alters the epigenome by bringing transcriptional activators, repressors and chromatin modifiers to the desired genomic region. This technique allows researchers to study how epigenetic marks like DNA methylation, histone acetylationacetylation and chromatin modifier proteins affect gene expression in cultured mammalian cells and live mice . Programmable gene regulation via Epigenetic CRISPR-Cas9 has potential clinical applications .

Edits on 31 Aug, 2018
Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 31 Aug, 2018
Edits made to:
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Juan Carlos Izpisua BelmonteJuan Carlos Izpisua Belmonte of the Salk Institute for Biological Studies, California and his team designed guide RNAs to bring transcriptional activators with dCas9 to activate genes of interest . Instead of fixing the mutated gene in a disease, their approach is to upregulate other genes in the disease pathway that can compensate for the malfunctioning gene. In mouse models for kidney disease and muscular dystrophy their approach improved kidney and muscle function.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 31 Aug, 2018
Edits made to:
Article (+15/-15 characters)
Article

Epigenetic CRISPR-Cas9 is a modified form of the genome editing platform CRISPR-Cas9 that does not cut DNA but affects gene expression levels. Gene expression levels depend on DNA methylationDNA methylation levels and whether chromatin conformation is in an active or repressed state. Epigenetic CRISPR-Cas9 uses the nuclease dead Cas9 (dCas9) to target the genome and instead of altering the genetic sequence, the system alters the epigenome by bringing transcriptional activators, repressors and chromatin modifiers to the desired genomic region. This technique allows researchers to study how epigenetic marks like DNA methylation, histone acetylation and chromatin modifier proteins affect gene expression in cultured mammalian cells and live mice . Programmable gene regulation via Epigenetic CRISPR-Cas9 has potential clinical applications .

Edits on 16 Aug, 2018
Carla Faraguna
Carla Faraguna edited on 16 Aug, 2018
Edits made to:
Further reading (+1 rows) (+3 cells) (+333 characters)
Further reading

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Jussi Taipale, Sandeep Botla, Emma Haapaniemi, Bernhard Schmierer, Jenna Persson

CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response

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