GoldenGolden
Advanced Search
CRISPR-Cas9

CRISPR-Cas9

CRISPR-Cas9 is a genome editing system. CRISPR systems provides immunity to bacteria and archaea from viruses and has been adapted for use as a genome editing tool capable of knocking out genes and rewriting genetic sequences in animal, plant and fungi. CRISPR-Cas9 is being adapted to other applications outside genome editing.

All edits by  Carla Faraguna 

Edits on 12 Nov, 2018
Carla Faraguna
Carla Faraguna edited on 12 Nov, 2018
Edits made to:
Table (+3 rows) (+12 cells) (+794 characters)
Table

Title
Author
Link
Type

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi

Christina S. Nødvig, Jakob B. Nielsen, Martin E. Kogle, Uffe H. Mortensen

Journal

A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans

Rebecca S Shapiro, Alejandro Chavez, Caroline B M Porter, Meagan Hamblin, Christian S Kaas, James E Dicarlo, Guisheng Zeng, Xiaoli Xu, Alexey V Revtovich, Natalia V Kirienko, Yue Wang, George M Church, James J Collins

Journal

Safeguarding CRISPR-Cas9 gene drives in yeast

James E Dicarlo, Alejandro Chavez, Sven L Dietz, Kevin M Esvelt, George M Church

Journal

Edits on 30 Oct, 2018
Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 30 Oct, 2018
Edits made to:
Article (+13/-13 characters)
Article

There is a patent dispute over the invention of CRISPR-Cas9 technology specifically for use in human cells, between Doudna’s research team and Feng Zhang’s group at the Broad Institute (MIT and Harvard) . Zhang’s research group and George ChurchGeorge Church’s lab at Harvard Medical School, each published Science papers in 2013 showing they had modified CRISPR-Cas9 to edit the genome in human and mouse cells . The Broad Institute’s US patent, the first of several for mammalian use of CRISPR, is under appeal . Citing lack of novelty, the European patent office has revoked the first patent obtained by the Broad Institute and has granted patents to the University of California and University of Vienna . The first is for using the CRISPR-Cas9 system across prokaryotic and eukaryotic systems and the second is for a modified form of CRISPR-Cas9 to regulate gene expression.

Carla Faraguna
Carla Faraguna edited on 30 Oct, 2018
Edits made to:
Table (+1 rows) (+3 cells) (+180 characters)
Table

Title
Author
Link
Type

Xconomy: Pre-Existing Immunity to CRISPR Found in 96% of People in Study

Web

Edits on 3 Oct, 2018
Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 3 Oct, 2018
Edits made to:
Article (+22/-22 characters)
Article

There is a patent dispute over the invention of CRISPR-Cas9 technology specifically for use in human cells, between Doudna’s research team and Feng Zhang’s group at the Broad Institute (MIT and Harvard) . Zhang’s research group and George Church’s lab at Harvard Medical SchoolHarvard Medical School, each published Science papers in 2013 showing they had modified CRISPR-Cas9 to edit the genome in human and mouse cells . The Broad Institute’s US patent, the first of several for mammalian use of CRISPR, is under appeal . Citing lack of novelty, the European patent office has revoked the first patent obtained by the Broad Institute and has granted patents to the University of California and University of Vienna . The first is for using the CRISPR-Cas9 system across prokaryotic and eukaryotic systems and the second is for a modified form of CRISPR-Cas9 to regulate gene expression.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 3 Oct, 2018
Edits made to:
Article (+18/-18 characters)
Article

Cas9 is the nuclease enzyme that does the cutting in the Type II CRISPR systems used by Streptococcus thermophilis . The function of Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) sequences that are part of a bacterial immune system, was discovered in the yogurt bacteria Streptococcus thermophilus . Phillipe Horvath and Rodolphe BarrangouRodolphe Barrangou of Danisco (later DuPont) made that discovery and in 2007 reported in Science that the bacteria incorporate sequences from phage viruses they have been exposed to as spacers in the CRISPR region which gave the bacteria resistance to those phage viruses . DuPont has patented a technique of exposing bacteria to different phage viruses and uses CRISPR sequences to tell them which ones have acquired resistance, something that helps them avoid phage viruses spoiling their yogurt .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 3 Oct, 2018
Edits made to:
Article (+15/-15 characters)
Article

Horvath and Barrangou teamed up with Virginjus Siksnys at Vilnius University, Lithuania and they published how CRISPR works with Cas9 in 2012 . Around the same time UC Berkeley’s Jennifer DoudnaJennifer Doudna and Emmanuelle Charpentier now at the Max Planck Institute for Infection Biology, Berlin also described in Science how Cas9 works in the CRISPR system . Because they also engineered a simpler version of CRISPR that could likely to work in other organisms including human cells , this transformed this bacterial immune function into a usable biotechnology tool.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 3 Oct, 2018
Edits made to:
Article (+15/-15 characters)
Article

CRISPR can fix the Cystic FibrosisCystic Fibrosis (CF) mutation in lung cells, intestinal cells, and iPS cells derived from patients . Editas Medicine and CRISPR Therapeutics are working towards a therapy to use on CF patients .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 3 Oct, 2018
Edits made to:
Article (+4/-4 characters)
Article

Cas9Cas9 is the nuclease enzyme that does the cutting in the Type II CRISPR systems used by Streptococcus thermophilis . The function of Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) sequences that are part of a bacterial immune system, was discovered in the yogurt bacteria Streptococcus thermophilus . Phillipe Horvath and Rodolphe Barrangou of Danisco (later DuPont) made that discovery and in 2007 reported in Science that the bacteria incorporate sequences from phage viruses they have been exposed to as spacers in the CRISPR region which gave the bacteria resistance to those phage viruses . DuPont has patented a technique of exposing bacteria to different phage viruses and uses CRISPR sequences to tell them which ones have acquired resistance, something that helps them avoid phage viruses spoiling their yogurt .

Edits on 29 Aug, 2018
Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+4/-4 characters)
Article

Targeting of Cas9 to cleave DNA in bacterial immune function uses two RNAsRNAs which form a duplex, the crRNA that recognizes the invading DNA and the tracrRNA which hybridizes with the crRNA . Doudna’s group engineered the system to use a single guide RNA . The CRISPR-Cas9 genome engineering system uses a single protein, Cas9, and single guide-RNA complex (Cas9-sgRNA) and it is the most commonly used CRISPR system for gene editing . The CRISPR-Cas9 user designed guide RNA binds DNA that contains the complementary sequence. The presence of a nearby (protospacer-adjacent motif) PAM sequence is required for cleavage in the target region. For endogenous CRISPR systems in bacteria, absence of PAM sequences in the bacteria’s own genome prevent self cleavage . In the human genome, the short PAM sequence is present at a frequency of 5.21% .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+9/-9 characters)
Article

Horvath and Barrangou teamed up with Virginjus Siksnys at Vilnius University, LithuaniaLithuania and they published how CRISPR works with Cas9 in 2012 . Around the same time UC Berkeley’s Jennifer Doudna and Emmanuelle Charpentier now at the Max Planck Institute for Infection Biology, Berlin also described in Science how Cas9 works in the CRISPR system . Because they also engineered a simpler version of CRISPR that could likely to work in other organisms including human cells , this transformed this bacterial immune function into a usable biotechnology tool.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+20/-20 characters)
Article

Editas Medicine is developing a CRISPR-Cas9 therapy for Leber Hereditary Amaurosis . Autosomal recessive and autosomal dominant Retinitis PigmentosaRetinitis Pigmentosa, choroidal neovascularization and Age-Related Macular Degeneration are forms of blindness that are being tested with CRISPR-Cas9 preclinically in animal models .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+26/-26 characters)
Article

Cas9 is the nuclease enzyme that does the cutting in the Type II CRISPR systems used by Streptococcus thermophilis . The function of Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) sequences that are part of a bacterial immune system, was discovered in the yogurt bacteria Streptococcus thermophilusStreptococcus thermophilus . Phillipe Horvath and Rodolphe Barrangou of Danisco (later DuPont) made that discovery and in 2007 and reported in Science that the bacteria incorporate sequences from phage viruses they have been exposed to as spacers in the CRISPR region which gave the bacteria resistance to those phage viruses . DuPont has patented a technique of exposing bacteria to different phage viruses and uses CRISPR sequences to tell them which ones have acquired resistance, something that helps them avoid phage viruses spoiling their yogurt .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+14/-14 characters)
Article

CRISPR-Cas9 is a genome editinggenome editing system. The system originates in bacteria providing immunity to viruses and has been adapted for use as a genome editing tool capable of knocking out genes and rewriting genetic sequences in animal, plant and fungi. Outside of genome editing, modifications to the CRISPR-Cas9 system make it useful for gene regulation, genome imaging and studying protein-genome interactions.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+6/-6 characters)
Article

Cas9 is the nuclease enzyme that does the cutting in the Type II CRISPRCRISPR systems used by Streptococcus thermophilis . The function of Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) sequences that are part of a bacterial immune system, was discovered in the yogurt bacteria Streptococcus thermophilus . Phillipe Horvath and Rodolphe Barrangou of Danisco (later DuPont) made that discovery and in 2007 and reported in Science that the bacteria incorporate sequences from phage viruses they have been exposed to as spacers in the CRISPR region which gave the bacteria resistance to those phage viruses . DuPont has patented a technique of exposing bacteria to different phage viruses and uses CRISPR sequences to tell them which ones have acquired resistance, something that helps them avoid phage viruses spoiling their yogurt .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+15/-15 characters)
Article

There is a patent dispute over the invention of CRISPR-Cas9 technology specifically for use in human cells, between Doudna’s research team and Feng Zhang’s group at the Broad InstituteBroad Institute (MIT and Harvard) . Zhang’s research group and George Church’s lab at Harvard Medical School, each published Science papers in 2013 showing they had modified CRISPR-Cas9 to edit the genome in human and mouse cells . The Broad Institute’s US patent, the first of several for mammalian use of CRISPR, is under appeal . Citing lack of novelty, the European patent office has revoked the first patent obtained by the Broad Institute and has granted patents to the University of California and University of Vienna . The first is for using the CRISPR-Cas9 system across prokaryotic and eukaryotic systems and the second is for a modified form of CRISPR-Cas9 to regulate gene expression.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+18/-18 characters)
Article

Horvath and Barrangou teamed up with Virginjus Siksnys at Vilnius UniversityVilnius University, Lithuania and they published how CRISPR works with Cas9 in 2012 . Around the same time UC Berkeley’s Jennifer Doudna and Emmanuelle Charpentier now at the Max Planck Institute for Infection Biology, Berlin also described in Science how Cas9 works in the CRISPR system . Because they also engineered a simpler version of CRISPR that could likely to work in other organisms including human cells , this transformed this bacterial immune function into a usable biotechnology tool.

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+3/-3 characters)
Article

Targeting of Cas9 to cleave DNADNA in bacterial immune function uses two RNAs which form a duplex, the crRNA that recognizes the invading DNA and the tracrRNA which hybridizes with the crRNA . Doudna’s group engineered the system to use a single guide RNA . The CRISPR-Cas9 genome engineering system uses a single protein, Cas9, and single guide-RNA complex (Cas9-sgRNA) and it is the most commonly used CRISPR system for gene editing . The CRISPR-Cas9 user designed guide RNA binds DNA that contains the complementary sequence. The presence of a nearby (protospacer-adjacent motif) PAM sequence is required for cleavage in the target region. For endogenous CRISPR systems in bacteria, absence of PAM sequences in the bacteria’s own genome prevent self cleavage . In the human genome, the short PAM sequence is present at a frequency of 5.21% .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+12/-12 characters)
Article

Targeting of Cas9 to cleave DNA in bacterial immune function uses two RNAs which form a duplex, the crRNA that recognizes the invading DNA and the tracrRNA which hybridizes with the crRNA . Doudna’s group engineered the system to use a single guide RNA . The CRISPR-Cas9 genome engineering system uses a single protein, Cas9, and single guide-RNA complex (Cas9-sgRNA) and it is the most commonly used CRISPR system for gene editing . The CRISPR-Cas9 user designed guide RNA binds DNA that contains the complementary sequence. The presence of a nearby (protospacer-adjacent motif) PAM sequence is required for cleavage in the target region. For endogenous CRISPR systems in bacteria, absence of PAM sequences in the bacteria’s own genome prevent self cleavage . In the human genomehuman genome, the short PAM sequence is present at a frequency of 5.21% .

Carla Faraguna
Carla Faraguna approved a suggestion from Golden's AI on 29 Aug, 2018
Edits made to:
Article (+15/-15 characters)
Article

There is a patent dispute over the invention of CRISPR-Cas9 technology specifically for use in human cells, between Doudna’s research team and Feng Zhang’s group at the Broad Institute (MIT and Harvard) . Zhang’s research group and George Church’s lab at Harvard Medical School, each published Science papers in 2013 showing they had modified CRISPR-Cas9 to edit the genome in human and mouse cells . The Broad Institute’s US patent, the first of several for mammalian use of CRISPR, is under appeal . Citing lack of novelty, the European patent office has revoked the first patent obtained by the Broad Institute and has granted patents to the University of California and University of Vienna . The first is for using the CRISPR-Cas9 system across prokaryotic and eukaryotic systems and the second is for a modified form of CRISPR-Cas9 to regulate gene expressiongene expression.

Carla Faraguna
Carla Faraguna edited on 28 Aug, 2018
Edits made to:
Description (+1 characters)
Topic thumbnail

CRISPR-Cas9

CRISPR-Cas9 is a genome editing system. The system originates in bacteria providing immunity to viruses and has been adapted for use as a genome editing tool capable of knocking out genes and rewriting genetic sequences in animal, plant and fungi. CRISPR-Cas9 is being adapted to other applications outside genome editing.

Golden logo
Text is available under the Creative Commons Attribution-ShareAlike 4.0; additional terms apply. By using this site, you agree to our Terms & Conditions.